LIN28B Antikörper

Produktnummer ABIN7091335

Der Maus Monoklonal Anti-LIN28B-Antikörper wurde für WB validiert. Er ist geeignet, LIN28B in Proben von Human zu detektieren.

Kurzübersicht für LIN28B Antikörper (ABIN7091335)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIN28B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: K7L3

Produktdetails anti-LIN28B Antikörper

Homologie: Human

Immunogen: Recombinant human LIN28B.

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Andere Bezeichnung: LIN28B

Hintergrund:

Synonyms: Lin-28B, LN28B_HUMAN, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B (C. elegans), Lin 28.2, Lin-28B, Protein lin-28 homolog B.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Gen-ID: 389421

UniProt: Q6ZN17