ORAI1 Antikörper (Extracellular)

Produktnummer ABIN7044554

Produktdetails anti-ORAI1 Antikörper

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Bindungsspezifität: Extracellular

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ORAI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Verwendungszweck: A Mouse Monoclonal Antibody to Human Orai1 Channel

Spezifität: Extracellular

Kreuzreaktivität (Details): Recognizes only human Orai1.

Homologie: Orai1 from human samples only

Produktmerkmale: Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1

Klon: 3F11-D10-B9

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Andere Bezeichnung: ORAI1 (ORAI1 Produkte)

Hintergrund: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Gen-ID: 84876

NCBI Accession: NM_032790

UniProt: Q96D31

Pathways: T-Zell Rezeptor Signalweg, BCR Signaling