NGFR Antikörper (Extracellular, Stalk Region)

Produktnummer ABIN7886728

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NGFR in WB, IHC, IF, FACS, IP, IC und LCI. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NGFR Antikörper (Extracellular, Stalk Region) (ABIN7886728)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunochromatography (IC), Live Cell Imaging (LCI)

Güteklasse: KO Validated

Produktdetails anti-NGFR Antikörper

Bindungsspezifität: AA 188-203, Extracellular, Stalk Region

Verwendungszweck: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, rat - 15,16 amino acid residues identical

Produktmerkmale: Anti-p75 NGF Receptor (extracellular) Antibody (ABIN7043344, ABIN7044755 and ABIN7044756) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP|IHC

Negative Control: (ABIN7236002)

Blocking Peptide: (ABIN7236002)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR

Hintergrund:

Synonyms: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Description: The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Gen-ID: 4804

NCBI Accession: NM_002507

UniProt: P08138

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding