NGFR Antikörper

Produktnummer ABIN6940192

Der Maus Monoklonal anti-NGFR Antikörper (Klon NGFR5) (ABIN6940192) detektiert spezifisch NGFR in IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Kaninchen, Cat, Frettchen, Affe und Pavian.

Kurzübersicht für NGFR Antikörper (ABIN6940192)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human, Kaninchen, Cat, Frettchen, Affe, Pavian

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: NGFR5

Produktdetails anti-NGFR Antikörper

Verwendungszweck: NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Antibody

Spezifität: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: NGFR from A875 melanoma cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma, Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR

Hintergrund:

CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor (NGFR), TNFR superfamily member 16, p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas., Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma

Molekulargewicht: 75kDa

Gen-ID: 4804, 415768, 681726

UniProt: P08138

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding