NGFR Antikörper

Produktnummer ABIN7044625

Der Maus Monoklonal anti-NGFR Antikörper wird verwendet zum Nachweis von NGFR in Proben von Ratte. Er wurde validiert für WB, IHC, IF und FACS.

Kurzübersicht für NGFR Antikörper (ABIN7044625)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 192-IgG

Produktdetails anti-NGFR Antikörper

Verwendungszweck: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR

Kreuzreaktivität (Details): 192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

Homologie: Recognizes p75NTR from rat samples only

Produktmerkmale: Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (ABIN7043342, ABIN7044624, ABIN7044625 and ABIN7044626) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only.

Aufreinigung: Affinity purified from cultured hybridoma medium.

Immunogen: Solubilized proteins from PC12 membranes.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

WB: 1:150-1:2500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 10 μL, 50 μL, 100 μL or 250 μL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR

Hintergrund:

Synonyms: p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Description: p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for b-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 Beta-amyloid peptide binds p75NTR and stimulates pro apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

Gen-ID: 24596

NCBI Accession: NM_002507

UniProt: Q08201

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding