EPH Receptor B4 Antikörper (Extracellular)

Produktnummer ABIN7043127

Produktdetails anti-EPH Receptor B4 Antikörper

Target: EPH Receptor B4 (EPHB4)

Bindungsspezifität: AA 472-486, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor B4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal Antibody to EphB4 Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Anti-EphB4 (extracellular) Antibody (ABIN7043127, ABIN7044253 and ABIN7044254)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize EphB4 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEKGAEGPSSVRFLK, corresponding to amino acid residues 472-486 of mouse EphB4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-ER024

Blocking Peptide: BLP-ER024

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Andere Bezeichnung: EPHB4 (EPHB4 Produkte)

Hintergrund: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK,EphB4 (Ephrin type-B receptor 4) is a member of the Eph family of receptor tyrosine kinases. Signaling pathways of these receptors play important roles in regulating numerous physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Ephrins transduce bidirectional signals based on their distribution on the communicating cell surfaces. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1,2.Eph receptors consist of two large subfamilies: EphAs and EphBs that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Each eph receptor may interact with multiple ligands, except for EPHB4, which only binds ephrin-B22.EPHB4 is expressed primarily during embryonic development in neural tissue and immature vasculature. Studies show that EphB4 is implicated in the growth of several epithelial tumors and is a promising target in cancer therapy. EphB4 signaling was found to promote tumor growth, invasiveness, chemoresistance and tumor angiogenesis3.

Alternative names: EphB4, Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK

Gen-ID: 13846

NCBI Accession: NM_004444

UniProt: P54761

Pathways: RTK Signalweg