C5AR1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7043082

Der Kaninchen Polyklonal anti-C5AR1 Antikörper (ABIN7043082) detektiert spezifisch C5AR1 in WB, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für C5AR1 Antikörper (Extracellular, N-Term) (ABIN7043082)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser C5AR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-C5AR1 Antikörper

Bindungsspezifität: AA 16-28, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor

Homologie: Human only Not recommended for use with rat,mouse samples

Produktmerkmale: Anti-Human C5aR1 (extracellular) Antibody (ABIN7043082 and ABIN7043927) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C5aR from human samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: C5AR1

Hintergrund:

Synonyms: C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88

Description: The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few.C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10.C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14.Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8.

Gen-ID: 728

NCBI Accession: NM_001736

UniProt: P21730

Pathways: Komplementsystem