C5AR1 Antikörper (N-Term)

Produktnummer ABIN1774738

Dieser Maus Monoklonal Antikörper detektiert spezifisch C5AR1 in WB, FACS, IHC (p) und IP. Es zeigt Reaktivität gegenüber Proben von Human, Rind (Kuh), Kaninchen und Frettchen. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für C5AR1 Antikörper (N-Term) (ABIN1774738)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human, Rind (Kuh), Kaninchen, Frettchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C5AR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon: S5-1

Produktdetails anti-C5AR1 Antikörper

Bindungsspezifität: AA 15-27, N-Term

Verwendungszweck: Anti-Hu CD88 Purified

Kreuzreaktivität (Details): Human, Bovine, Ferret, Rabbit

Produktmerkmale: The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 3-12 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-C5AR1 Antikörper (ABIN1774738)

Conway Morris, Kefala, Wilkinson, Dhaliwal, Farrell, Walsh, Mackenzie, Reid, Davidson, Haslett, Rossi, Sallenave, Simpson: "C5a mediates peripheral blood neutrophil dysfunction in critically ill patients." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 1, pp. 19-28, (2009) (PubMed).

Cain, Monk: "The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74)." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7165-9, (2002) (PubMed).

Kiener, Baghestanian, Dominkus, Walchshofer, Ghannadan, Willheim, Sillaber, Graninger, Smolen, Valent: "Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis." in: Arthritis and rheumatism, Vol. 41, Issue 2, pp. 233-45, (1998) (PubMed).

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: CD88

Hintergrund: Complement C5a receptor 1,CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.,C5A, C5R1, C5AR1

Gen-ID: 728

UniProt: P21730

Pathways: Komplementsystem