Calnexin Antikörper (Intracellular)

Produktnummer ABIN7043021

Der Kaninchen Polyklonal Anti-Calnexin-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, Calnexin in Proben von Ratte zu detektieren.

Kurzübersicht für Calnexin Antikörper (Intracellular) (ABIN7043021)

Target: Calnexin (CANX)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Calnexin Antikörper

Bindungsspezifität: AA 572-587, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Calnexin

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Calnexin Antibody (ABIN7043021, ABIN7044212 and ABIN7044213) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400-1:600

Kommentare:

Negative Control: (ABIN7235025)

Blocking Peptide: (ABIN7235025)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: CANX

Hintergrund: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90,Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Alternative names: Calnexin, Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Gen-ID: 29144

NCBI Accession: NM_001024649

UniProt: P35565

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis