ATP2A2 Antikörper (AA 498-510)

Produktnummer ABIN7042954

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATP2A2 in WB, IF und IC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für ATP2A2 Antikörper (AA 498-510) (ABIN7042954)

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP2A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-ATP2A2 Antikörper

Bindungsspezifität: AA 498-510

Verwendungszweck: A Rabbit Polyclonal Antibody to SERCA2 Pump

Spezifität: 2nd cytoplasmic loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-SERCA2 Antibody is directed against an epitope of rat SERCA2. Anti-SERCA2 Antibody (ABIN7042954, ABIN7044151 and ABIN7044152) can be used in western blot and immunocytochemistry applications and was designed to recognize SERCA2 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7234817)

Blocking Peptide: (ABIN7234817)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ATP2A2

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Andere Bezeichnung: ATP2A2

Hintergrund: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2,Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.

Alternative names: SERCA2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2

Gen-ID: 29693

NCBI Accession: NM_001681

UniProt: P11507

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process