EGF Antikörper (AA 609-751)

Produktnummer ABIN7428500

Der Maus Monoklonal Anti-EGF-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, EGF in Proben von Human zu detektieren.

Kurzübersicht für EGF Antikörper (AA 609-751) (ABIN7428500)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: D2

Produktdetails anti-EGF Antikörper

Bindungsspezifität: AA 609-751

Verwendungszweck: Monoclonal Antibody to Epidermal Growth Factor (EGF)

Spezifität: The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Kreuzreaktivität: Schwein

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Epidermal Growth Factor (EGF) corresdonding to Ala609~Gly751 with N-terminal His Tag

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Epidermal Growth Factor

Hintergrund: URG, Beta-Urogastrone, Pro-epidermal growth factor

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation