P4HB Antikörper (AA 190-240)

Produktnummer ABIN6992148

Der Kaninchen Polyklonal anti-P4HB Antikörper (ABIN6992148) detektiert spezifisch P4HB in WB, ELISA, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für P4HB Antikörper (AA 190-240) (ABIN6992148)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P4HB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-P4HB Antikörper

Bindungsspezifität: AA 190-240

Spezifität: PDIA1 antibody is human, mouse and rat reactive.

Aufreinigung: PDIA1 antibody is affinity chromatography purified via peptide column.

Immunogen: PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1. The immunogen is located within amino acids 190 - 240 of PDIA1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PDIA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PDIA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDIA1

Hintergrund: PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2). PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3). Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4).

Molekulargewicht: Predicted: 54 kDa

Observed: 54 kDa

Gen-ID: 5034

NCBI Accession: NP_000909

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism