PRAS40 Antikörper (C-Term)

Produktnummer ABIN6991875

Dieses Anti-PRAS40-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PRAS40 in WB, ELISA, IHC (p) und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für PRAS40 Antikörper (C-Term) (ABIN6991875)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRAS40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-PRAS40 Antikörper

Bindungsspezifität: AA 190-240, C-Term

Spezifität: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.

Aufreinigung: AKT1S1 Antibody is affinity chromatography purified via peptide column.

Immunogen: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: AKT1S1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Andere Bezeichnung: AKT1S1

Hintergrund: AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).

Molekulargewicht: Predicted: 30 kDa

Observed: 28 kDa

Gen-ID: 84335

NCBI Accession: NP_115751

UniProt: Q96B36

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt