ATG5 Antikörper (N-Term)

Produktnummer ABIN6991065

Dieses Anti-ATG5-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von ATG5 in WB, ELISA und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für ATG5 Antikörper (N-Term) (ABIN6991065)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG5 Antikörper

Bindungsspezifität: N-Term

Spezifität: Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.

Aufreinigung: ATG5 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5.

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μ,g/mL, IHC-P: 2.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ATG5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: ATG5

Hintergrund: ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

Molekulargewicht: Predicted: 32kD

Observed: 55kD (ATG5-ATG12 conjugation) kDa

Gen-ID: 9474

UniProt: A9UGY9

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie