AIF Antikörper (C-Term)

Produktnummer ABIN6990607

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AIF in WB, ELISA und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für AIF Antikörper (C-Term) (ABIN6990607)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AIF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-AIF Antikörper

Bindungsspezifität: C-Term

Spezifität: Multiple isoforms of AIF are known to exist.

Aufreinigung: AIF Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-AIF antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AIF. The immunogen is located within the last 50 amino acids of AIF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5-2 μ,g/mL, ICC: 5 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: AIF Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: AIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Andere Bezeichnung: AIF

Hintergrund: AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.

Molekulargewicht: Predicted: 67kD

Observed: 68kD kDa

Gen-ID: 10256

UniProt: O95381

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effekt