ORAI1 Antikörper (C-Term)

Produktnummer ABIN6990200

Der Maus Monoklonal anti-ORAI1 Antikörper wird verwendet zum Nachweis von ORAI1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF und IHC (p).

Kurzübersicht für ORAI1 Antikörper (C-Term) (ABIN6990200)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ORAI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 3F6H5

Produktdetails anti-ORAI1 Antikörper

Bindungsspezifität: C-Term

Spezifität: ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.

Aufreinigung: ORAI1 Monoclonal Antibody is Protein A purified.

Immunogen: Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.

Details zu ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Andere Bezeichnung: ORAI1

Hintergrund: ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Molekulargewicht: Predicted: 33 kDa

Observed: 56 kDa

Gen-ID: 84876

UniProt: Q96D31

Pathways: T-Zell Rezeptor Signalweg, BCR Signaling