PRAS40 Antikörper (AA 161-256)

Produktnummer ABIN6989135

Der Kaninchen Polyklonal Anti-PRAS40-Antikörper wurde für WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) und ICC validiert. Er ist geeignet, PRAS40 in Proben von Human zu detektieren.

Kurzübersicht für PRAS40 Antikörper (AA 161-256) (ABIN6989135)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRAS40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Produktdetails anti-PRAS40 Antikörper

Bindungsspezifität: AA 161-256

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Cow,Sheep,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PRAS40

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Andere Bezeichnung: PRAS40

Hintergrund:

Synonyms: AKT1 substrate 1 (proline rich), AKT1 substrate 1, AKT1-S1, AKT1S-1, AKT1S 1, AKT1S1, Lobe, PRAS 40, PRAS-40, PRAS40, Proline rich akt substrate, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, AKT1 S1, AKT1 substrate 1, AKTS1_HUMAN, MGC2865, PRAS40, Proline rich Akt substrate 40 kDa, Proline-rich AKT1 substrate 1.

Background: Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Gen-ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt