CD25 Antikörper

Produktnummer ABIN6961442

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch CD25 in FACS, IP, IHC (fro) und Func. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für CD25 Antikörper (ABIN6961442)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Klon: PC61-5

Produktdetails anti-CD25 Antikörper

Aufreinigung: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Endotoxin-Niveau: Low endotoxin: < 0.01 EU/ug

Isotyp: IgG1 lambda

Anwendungsinformationen

Applikationshinweise: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Kommentare:

2 mg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25

Hintergrund: The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL- 2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25-.

Gen-ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation