Rekombinanter RFP Antikörper (Alexa Fluor 647)

Produktnummer ABIN6953234

Dieses Anti-RFP-Antikörper ist ein Alpaca Monoklonal-Antikörper zur Detektion von RFP in IF, FACS und ICC. Geeignet für Discosoma.

Kurzübersicht für Rekombinanter RFP Antikörper (Alexa Fluor 647) (ABIN6953234)

Target: RFP (Red Fluorescent Protein (RFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Discosoma

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 2B12

Produktdetails anti-RFP Antikörper (Alexa Fluor 647)

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-RFP conjugated with Alexa647, Clone 2B12

Spezifität: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Kreuzreaktivität (Details): Does not cross-react with GFP or mTagBFP derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Konzentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP