WEE1 Antikörper (AA 151-250) (AbBy Fluor® 350)

Produktnummer ABIN6945379

Der Kaninchen Polyklonal Anti-WEE1-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, WEE1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für WEE1 Antikörper (AA 151-250) (AbBy Fluor® 350) (ABIN6945379)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WEE1 Antikörper ist konjugiert mit AbBy Fluor® 350

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-WEE1 Antikörper (AbBy Fluor® 350)

Bindungsspezifität: AA 151-250

Kreuzreaktivität: Human, Ratte

Homologie: Mouse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WEE1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Andere Bezeichnung: WEE1

Hintergrund:

Synonyms: DKFZp686I18166, EC 2.7.10.2, FLJ16446, Wee 1, WEE-1, WEE1 protein, Wee1 homolog, WEE1 homolog S. pombe, Wee1 like protein kinase, Wee1 tyrosine kinase, Wee1+ homolog, Wee1+ S. pombe homolog, WEE1A, Wee1A kinase, WEE1hu, WEE1 tyrosine kinase isoform 1, MGC105683, WEE1_HUMAN.

Background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gen-ID: 7465

UniProt: P30291

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase