PCNA Antikörper

Produktnummer ABIN6944453

Produktdetails anti-PCNA Antikörper

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant human PCNA between 100 to 200 amino acids

Klon: 1G3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA (PCNA Produkte)

Synonyme: PCNAR antikoerper, Pcna/cyclin antikoerper, pcna antikoerper, AO090003000846 antikoerper, 53/13 antikoerper, CG9193 antikoerper, DmPCNA antikoerper, Dmel\\CG9193 antikoerper, MUS209 antikoerper, Mus209 antikoerper, PCNA antikoerper, PCNA/mus209 antikoerper, Pcna antikoerper, dPCNA antikoerper, l(2)02448 antikoerper, l(2)56F antikoerper, l(2)56Fa antikoerper, l(2)s1534 antikoerper, cb16 antikoerper, fb36g03 antikoerper, wu:fa28e03 antikoerper, wu:fb36g03 antikoerper, pcna-A antikoerper, proliferating cell nuclear antigen antikoerper, DNA polymerase III sliding clamp antikoerper, Proliferating cell nuclear antigen antikoerper, Proliferating cell nuclear antigen-like antikoerper, proliferating cell nuclear antigen L homeolog antikoerper, proliferating cell nuclear antigen 1 antikoerper, PCNA antikoerper, Pcna antikoerper, pcna antikoerper, MMP_RS08810 antikoerper, ANI_1_512014 antikoerper, AOR_1_1500154 antikoerper, MGC53867 antikoerper, pcna.L antikoerper, pcna1 antikoerper, LOC106420145 antikoerper, pcn1 antikoerper

Hintergrund:

Synonyms: ProlifeRating cell nuclear antigen, Cyclin, PCNA

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie