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Keratin 77 Antikörper

Der Maus Monoklonal Anti-Keratin 77-Antikörper wurde für IHC und StM validiert. Er ist geeignet, Keratin 77 in Proben von Human und Ratte zu detektieren.
Produktnummer ABIN6941447

Kurzübersicht für Keratin 77 Antikörper (ABIN6941447)

Target

Alle Keratin 77 (KRT77) Antikörper anzeigen
Keratin 77 (KRT77)

Reaktivität

  • 19
  • 6
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte

Wirt

  • 12
  • 7
Maus

Klonalität

  • 10
  • 9
Monoklonal

Konjugat

  • 15
  • 2
  • 1
  • 1
Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation

  • 11
  • 9
  • 7
  • 6
  • 5
  • 3
  • 2
  • 1
Immunohistochemistry (IHC), Staining Methods (StM)

Klon

KRTL-1077
  • Spezifität

    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

    Kreuzreaktivität (Details)

    Shows broad species reactivity.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant human KRT77 protein

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Positive Control: Skin, Squamous cell carcinoma (SCC).

    Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    Keratin 77 (KRT77)

    Andere Bezeichnung

    KRT77

    Molekulargewicht

    56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

    Gen-ID

    374454

    UniProt

    Q7Z794
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