CDK1 Antikörper (C-Term)

Produktnummer ABIN6941282

Dieser Anti-CDK1-Antikörper ist ein Maus-Monoklonal-Antikörper zum Nachweis von CDK1 in WB, IHC, IF, IP, Act, KAA und StM. Geeignet für Human, Maus, Ratte, Huhn, Xenopus laevis, Meerschweinchen und Marmota monax.

Kurzübersicht für CDK1 Antikörper (C-Term) (ABIN6941282)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Maus, Ratte, Huhn, Xenopus laevis, Meerschweinchen, Marmota monax

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Activation (Act), Kinase Activity Assay (KAA), Staining Methods (StM)

Klon: A17-1-1

Produktdetails anti-CDK1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes a 34 kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Aufreinigung: Purified by Protein A/G

Immunogen: C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Human tonsil (IHC).

Known Application: Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence (1-2 μg/mL), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2

Molekulargewicht: 34kDa

Gen-ID: 983

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA