ZAP70 Antikörper (AA 247-382)

Produktnummer ABIN6940914

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ZAP70 in IHC, ELISA, StM und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ZAP70 Antikörper (AA 247-382) (ABIN6940914)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Klon: ZAP70-2046

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: AA 247-382

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 247-382) of human ZAP70 protein (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells. Tonsil or lymph node.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Molekulargewicht: 70kDa

Gen-ID: 7535

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway