Rekombinanter Ubiquitin B Antikörper (AA 1-119)

Produktnummer ABIN6940846

Der Kaninchen Monoklonal Anti-Ubiquitin B-Antikörper wurde für IHC und StM validiert. Er ist geeignet, Ubiquitin B in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Ubiquitin B Antikörper (AA 1-119) (ABIN6940846)

Target: Ubiquitin B (UBB)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: UBB-3143R

Produktdetails anti-Ubiquitin B Antikörper

Bindungsspezifität: AA 1-119

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or Jurkat cells. Alzheimer's Brain.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Ubiquitin B

Target: Ubiquitin B (UBB)

Andere Bezeichnung: UBB

Hintergrund: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Molekulargewicht: 9kDa

Gen-ID: 7314

UniProt: P62979, P62987, P62988

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagie, EGFR Downregulation, Ubiquitin Proteasome Pathway