PCNA Antikörper

Produktnummer ABIN6940267

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PCNA in WB, IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Saccharomyces cerevisiae.

Kurzübersicht für PCNA Antikörper (ABIN6940267)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: PC5

Produktdetails anti-PCNA Antikörper

Spezifität: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Aufreinigung: Purified by Protein A/G

Immunogen: Rat PCNA/protein A fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Molekulargewicht: 36kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie