PCNA Antikörper

Produktnummer ABIN6940267

Dieser Maus Monoklonal Antikörper erkennt spezifisch PCNA in WB, IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Saccharomyces cerevisiae.

Kurzübersicht für PCNA Antikörper (ABIN6940267)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: PC5

Produktdetails anti-PCNA Antikörper

Spezifität: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Aufreinigung: Purified by Protein A/G

Immunogen: Rat PCNA/protein A fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Molekulargewicht: 36kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie