PCNA Antikörper

Produktnummer ABIN6940266

Der Maus Monoklonal anti-PCNA Antikörper (Klon PCNA-694) (ABIN6940266) detektiert spezifisch PCNA in WB, IHC, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für PCNA Antikörper (ABIN6940266)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: PCNA-694

Produktdetails anti-PCNA Antikörper

Verwendungszweck: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Spezifität: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Kreuzreaktivität (Details): Shows broad species reactivity.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full length human PCNA protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Tonsil or reactive lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund:

Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

Tonsil or reactive lymph node.

Molekulargewicht: 36kDa

Gen-ID: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie