OGG1 Antikörper

Produktnummer ABIN6940242

Dieser Maus Monoklonal Antikörper detektiert spezifisch OGG1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für OGG1 Antikörper (ABIN6940242)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OGG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-OGG1-1

Produktdetails anti-OGG1 Antikörper

Verwendungszweck: 8-Oxoguanine DNA Glycosylase Antibody

Spezifität: 8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human OGG1 protein

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or Jurkat cells. Kidney, skin or lymph node.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Andere Bezeichnung: OGG1

Hintergrund:

HMMH, HOGG1, MUTM, OGH1

HeLa or Jurkat cells. Kidney, skin or lymph node, Daudi, MDA-MB-231

Molekulargewicht: 39kDa

Gen-ID: 4968, 380271

UniProt: O15527

Pathways: DNA Reparatur