Cytokeratin 18 Antikörper

Produktnummer ABIN6939935

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cytokeratin 18 in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund, Schwein, Cat, Rind (Kuh), Hamster, Schaf, Pferd, Frettchen, Ziege und Marmoset.

Kurzübersicht für Cytokeratin 18 Antikörper (ABIN6939935)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Cat, Rind (Kuh), Hamster, Schaf, Pferd, Frettchen, Ziege, Marmoset

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: C-04-same as Ks18-04

Produktdetails anti-Cytokeratin 18 Antikörper

Verwendungszweck: Cytokeratin 18 (KRT18) Antibody

Spezifität: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Keine Kreuzreaktivität: Huhn, Xenopus laevis

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: KRT18

Hintergrund:

Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC).

Molekulargewicht: 45kDa

Gen-ID: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose