Cytokeratin 18 Antikörper

Produktnummer ABIN6939932

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cytokeratin 18 in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Cytokeratin 18 Antikörper (ABIN6939932)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: KRT18-834

Produktdetails anti-Cytokeratin 18 Antikörper

Verwendungszweck: Cytokeratin 18 (KRT18) Antibody

Spezifität: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Keine Kreuzreaktivität: Rind (Kuh), Hund, Hamster, Maus, Schwein, Ratte (Rattus), Schaf

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human KRT18 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HCT116, MCF-7, HeLa, A431 cells. Stomach, Intestine or Breast Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: KRT18

Hintergrund:

Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

A431 cells. Stomach, HCT116, HeLa, Intestine or Breast Carcinoma., MCF-7

Molekulargewicht: 45kDa

Gen-ID: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose