Ubiquitin B Antikörper (AA 1-119)

Produktnummer ABIN6938717

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Ubiquitin B in WB, IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Ubiquitin B Antikörper (AA 1-119) (ABIN6938717)

Target: Ubiquitin B (UBB)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: UBB-1748

Produktdetails anti-Ubiquitin B Antikörper

Bindungsspezifität: AA 1-119

Spezifität: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Isotyp: IgG2c kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Ubiquitin B

Target: Ubiquitin B (UBB)

Andere Bezeichnung: UBB

Hintergrund: Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2,Ubiquitin (Autophagy Marker)
Cellular localisation: Cell Surface, Cytoplasmic and Nuclear

Molekulargewicht: 9kDa

Gen-ID: 7314, 356190, 520348

UniProt: P62979, P62987, P62988

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagie, EGFR Downregulation, Ubiquitin Proteasome Pathway