SHP1 Antikörper

Produktnummer ABIN6938266

Dieses Anti-SHP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SHP1 in WB, IF, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für SHP1 Antikörper (ABIN6938266)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SHP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CPTC-APEX1-2

Produktdetails anti-SHP1 Antikörper

Spezifität: APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length APEX1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431, A549, PC3, HAP1, HePG2, MCF-7, HeLa, NIH/3T3 and C6 whole cell lysates. Human ovarian carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Andere Bezeichnung: PTPN6

Hintergrund: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APEX nuclease, Redox factor-1, APX, protein REF-1,APEX Nuclease I
Cellular localisation: Mitochondrion. Nucleus.

Molekulargewicht: 35kDa

Gen-ID: 328, 73722

UniProt: P27695

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling