PCNA Antikörper
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- Target Alle PCNA Antikörper anzeigen
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser PCNA Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
- Kreuzreaktivität (Details)
- Human. Dog. Shows broad species reactivity.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full length human PCNA protein
- Klon
- PCNA-694
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product PCNA Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil or reactive lymph node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Andere Bezeichnung
- PCNA (PCNA Produkte)
- Synonyme
- PCNAR antikoerper, Pcna/cyclin antikoerper, pcna antikoerper, AO090003000846 antikoerper, 53/13 antikoerper, CG9193 antikoerper, DmPCNA antikoerper, Dmel\\CG9193 antikoerper, MUS209 antikoerper, Mus209 antikoerper, PCNA antikoerper, PCNA/mus209 antikoerper, Pcna antikoerper, dPCNA antikoerper, l(2)02448 antikoerper, l(2)56F antikoerper, l(2)56Fa antikoerper, l(2)s1534 antikoerper, cb16 antikoerper, fb36g03 antikoerper, wu:fa28e03 antikoerper, wu:fb36g03 antikoerper, pcna-A antikoerper, proliferating cell nuclear antigen antikoerper, DNA polymerase III sliding clamp antikoerper, Proliferating cell nuclear antigen antikoerper, Proliferating cell nuclear antigen-like antikoerper, proliferating cell nuclear antigen L homeolog antikoerper, proliferating cell nuclear antigen 1 antikoerper, PCNA antikoerper, Pcna antikoerper, pcna antikoerper, MMP_RS08810 antikoerper, ANI_1_512014 antikoerper, AOR_1_1500154 antikoerper, MGC53867 antikoerper, pcna.L antikoerper, pcna1 antikoerper, LOC106420145 antikoerper, pcn1 antikoerper
- Hintergrund
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Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein,PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)
Cellular localisation: Predominantly nuclear, some cytoplasmic - Molekulargewicht
- 36kDa
- Gen-ID
- 5111, 147433, 728886
- UniProt
- P12004
- Pathways
- Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie
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