MCM6 Antikörper (AA 228-368)

Produktnummer ABIN6937817

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MCM6 in IHC und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MCM6 Antikörper (AA 228-368) (ABIN6937817)

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: MCM6-2999

Produktdetails anti-MCM6 Antikörper

Bindungsspezifität: AA 228-368

Spezifität: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human skin or squamous cell carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MCM6

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Andere Bezeichnung: MCM6

Hintergrund: DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM,MCM6 (Proliferation Marker)
Cellular localisation: Nuclear

Molekulargewicht: 105kDa

Gen-ID: 4175, 444118

UniProt: Q14566

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA