Keratin 5/8 Antikörper
Kurzübersicht für Keratin 5/8 Antikörper (ABIN6937713)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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Keine Kreuzreaktivität
- Huhn, Xenopus laevis
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Kreuzreaktivität (Details)
- Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
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Immunogen
- Cytoskeletal preparation from HeLa cells
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Isotyp
- IgG1 kappa
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Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Konservierungsmittel
- Azide free
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2 to 8°C. Antibody is stable for 24 months.
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Haltbarkeit
- 24 months
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- Keratin 5/8 (KRT5/8)
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Andere Bezeichnung
- KRT5 & KRT8
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Hintergrund
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CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
Cellular localisation: Cytoplasmic -
Molekulargewicht
- 58kDa (CK5), 52.5kDa (CK8)
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Gen-ID
- 3852, 3856, 433845
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UniProt
- P13647, P05787
Target
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