Keratin 5/8 Antikörper (AA 353-367) (Biotin)

Produktnummer ABIN344035

Produktdetails anti-Keratin 5/8 Antikörper (Biotin)

Target: Keratin 5/8 (KRT5/8)

Bindungsspezifität: AA 353-367

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 5/8 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Spezifität: Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

Aufreinigung: Protein A purified

Immunogen: Human cytokeratin 8, purified from SDS PAGE.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Details zu Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Andere Bezeichnung: Cytokeratin 5+8 (KRT5/8 Produkte)

Hintergrund: This antibody detects: Cytokeratin 5+8