Keratin 5/8 Antikörper

Produktnummer ABIN6941336

Produktdetails anti-Keratin 5/8 Antikörper

Target: Keratin 5/8 (KRT5/8)

Reaktivität: Human, Maus, Schwein, Rind (Kuh), Ratte, Hund, Hamster, Schaf, Frettchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 5/8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Keine Kreuzreaktivität: Huhn, Xenopus laevis

Aufreinigung: Purified by Protein A/G

Immunogen: Cytoskeletal preparation from HeLa cells

Klon: C-50

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 to 8°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Details zu Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Andere Bezeichnung: KRT5 & KRT8 (KRT5/8 Produkte)

Molekulargewicht: 58kDa (CK5), 52.5kDa (CK8)

Gen-ID: 3852, 3856

UniProt: P13647, P05787