Keratin 5/8 Antikörper

Produktnummer ABIN3025013

Produktdetails anti-Keratin 5/8 Antikörper

Target: Keratin 5/8 (KRT5/8)

Reaktivität: Human, Maus, Schwein, Rind (Kuh), Ratte, Hund, Hamster, Schaf, Frettchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 5/8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Keine Kreuzreaktivität: Huhn, Xenopus laevis

Produktmerkmale: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Aufreinigung: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

Klon: SPM268

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Andere Bezeichnung: Cytokeratin 5/8 (KRT5/8 Produkte)

Hintergrund: It reacts with keratin 5 (58  kDa) and keratin 8 (52.5  kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.