Dystrophin Antikörper (AA 114-263)

Produktnummer ABIN6937092

Der Maus Monoklonal Anti-Dystrophin-Antikörper wurde für IHC, ELISA, Coat und IHC (f) validiert. Er ist geeignet, Dystrophin in Proben von Human zu detektieren.

Kurzübersicht für Dystrophin Antikörper (AA 114-263) (ABIN6937092)

Target: Dystrophin (DMD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dystrophin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: DMD-3242

Produktdetails anti-Dystrophin Antikörper

Bindungsspezifität: AA 114-263

Spezifität: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human skeletal muscle and heart muscle tissues (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Dystrophin

Target: Dystrophin (DMD)

Andere Bezeichnung: DMD

Hintergrund: BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types,Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 427kDa

Gen-ID: 1756, 495912

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development