Dystrophin Antikörper (AA 114-263)

Produktnummer ABIN6939242

Der Maus Monoklonal anti-Dystrophin Antikörper wird verwendet zum Nachweis von Dystrophin in Proben von Human. Er wurde validiert für IHC und ELISA.

Kurzübersicht für Dystrophin Antikörper (AA 114-263) (ABIN6939242)

Target: Dystrophin (DMD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dystrophin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Klon: DMD-3242

Produktdetails anti-Dystrophin Antikörper

Bindungsspezifität: AA 114-263

Verwendungszweck: Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy) Antibody

Spezifität: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human skeletal muscle and heart muscle tissues (IHC).

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Dystrophin

Target: Dystrophin (DMD)

Andere Bezeichnung: DMD

Hintergrund:

BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types

Human skeletal muscle and heart muscle tissues (IHC).

Molekulargewicht: 427kDa

Gen-ID: 1756, 495912

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development