CDKN1B Antikörper

Produktnummer ABIN6936909

Der Maus Monoklonal anti-CDKN1B Antikörper wird verwendet zum Nachweis von CDKN1B in Proben von Human, Maus, Ratte und Affe. Er wurde validiert für IHC und IF.

Kurzübersicht für CDKN1B Antikörper (ABIN6936909)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: SPM348

Produktdetails anti-CDKN1B Antikörper

Verwendungszweck: p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody

Spezifität: This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Kreuzreaktivität (Details): Human. Mouse. Rat. Monkey.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified GST-p27 fusion protein of Mouse origin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: ZR75, T47D, SK-BR-3, MDA-MB-231, MCF7 cells. Tonsil, Breast or Colon Ca.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: CDKN1B

Hintergrund:

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Breast or Colon Ca., MCF7 cells. Tonsil, MDA-MB-231, SK-BR-3, T47D, ZR75

Molekulargewicht: 25-26kDa

Gen-ID: 1027, 238990

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie