CDK2 Antikörper

Produktnummer ABIN6936897

Der Maus Monoklonal Anti-CDK2-Antikörper wurde für WB, IP, FACS, Act und KAA validiert. Er ist geeignet, CDK2 in Proben von Human zu detektieren.

Kurzübersicht für CDK2 Antikörper (ABIN6936897)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Activation (Act), Kinase Activity Assay (KAA)

Klon: AN4-3

Produktdetails anti-CDK2 Antikörper

Spezifität: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.

Kreuzreaktivität (Details): Human. Mouse. Xenopus.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Cdk2 protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Inhibits activation of p34cdc2 kinase by cyclins, Immunoprecipitation, Kinase Assay, ,Western Blot, Flow cytometry (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. HeLa whole cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Andere Bezeichnung: CDK2

Hintergrund: Cdc2 related protein kinase, CDC28, CDC2A, CDK1, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, cyclin dependent kinase 2 alpha, kinase Cdc2, MPF, p33 protein kinase,Cdk2 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear

Molekulargewicht: 34kDa

Gen-ID: 1017, 19192

UniProt: P24941

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA