CDK1 Antikörper (C-Term)

Produktnummer ABIN6936870

Der Maus Monoklonal Anti-CDK1-Antikörper wurde für WB, IHC, IF, IP, IHC (f), Act und KAA validiert. Er ist geeignet, CDK1 in Proben von Xenopus laevis zu detektieren.

Kurzübersicht für CDK1 Antikörper (C-Term) (ABIN6936870)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Activation (Act), Kinase Activity Assay (KAA)

Klon: A17-1-1

Produktdetails anti-CDK1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes a 34 kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Kreuzreaktivität (Details): Human. Mouse. Rat. Guinea Pig. Woodchuck. Xenopus. Chicken.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence (1-2 μg/mL), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human tonsil (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2

Hintergrund: Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2,Cdk1 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear and Cytoplasmic

Molekulargewicht: 34kDa

Gen-ID: 983, 334562

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA