Calnexin Antikörper (AA 1-300)

Produktnummer ABIN6936576

Der Maus Monoklonal Anti-Calnexin-Antikörper wurde für WB, ELISA, Coat und IHC (f) validiert. Er ist geeignet, Calnexin in Proben von Human zu detektieren.

Kurzübersicht für Calnexin Antikörper (AA 1-300) (ABIN6936576)

Target: Calnexin (CANX)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CANX-1541

Produktdetails anti-Calnexin Antikörper

Bindungsspezifität: AA 1-300

Spezifität: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: CANX

Hintergrund: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90,Calnexin (Endoplasmic Reticulum Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 90kDa

Gen-ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis