Rekombinanter BCL10 Antikörper (AA 122-168)

Produktnummer ABIN6936471

Der Kaninchen Monoklonal Anti-BCL10-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, BCL10 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter BCL10 Antikörper (AA 122-168) (ABIN6936471)

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BCL10 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: BL10-2988R

Produktdetails anti-BCL10 Antikörper

Bindungsspezifität: AA 122-168

Spezifität: BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1,14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NF B activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1,14) translocation, while 55 % of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human BCL10 protein fragment (around aa122-168) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: K562 or HepG2 cells. Human lymphoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu BCL10

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Andere Bezeichnung: BCL10

Hintergrund: B-cell CLL/lymphoma 10, B-cell leukemia/lymphoma 10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, caspase-recruiting domain-containing protein, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, Cellular-E10 (cE10), CIPER, hCLAP, mE10, R-RCD1,BCL10 (MALT-Lymphoma Marker)
Cellular localisation: Nucleus. Cytoplasm.

Molekulargewicht: 33kDa

Gen-ID: 8915, 193516

UniProt: O95999

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine