Rekombinanter AMACR Antikörper (AA 297-394)

Produktnummer ABIN6936406

Produktdetails anti-AMACR Antikörper

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 297-394

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser AMACR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and i²-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)

Klon: AMACR-2748R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK cells. Human prostate adenocarcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: AMACR (AMACR Produkte)

Synonyme: AMACR antikoerper, DKFZp469O1232 antikoerper, amacr antikoerper, NCU04099.1 antikoerper, MGC89832 antikoerper, AMACRD antikoerper, CBAS4 antikoerper, RACE antikoerper, RM antikoerper, Macr1 antikoerper, Da1-8 antikoerper, Marc1 antikoerper, alpha-methylacyl-CoA racemase antikoerper, PROBABLE ALPHA-METHYLACYL-COA RACEMASE MCR (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) antikoerper, Alpha-methylacyl-CoA racemase antikoerper, alpha-methylacyl-CoA racemase L homeolog antikoerper, AMACR antikoerper, mcr antikoerper, BPSL0064 antikoerper, Maqu_2037 antikoerper, UREG_03852 antikoerper, MCYG_01809 antikoerper, amacr antikoerper, amacr.L antikoerper, MGYG_04259 antikoerper, NCU04099 antikoerper, Amacr antikoerper

Hintergrund: Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM,AMACR / p504S (Prostate Cancer Marker)
Cellular localisation: Cytoplasmic (granular)

Molekulargewicht: 42kDa

Gen-ID: 23600, 508343

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process