Rekombinanter AMACR Antikörper (AA 297-394)

Produktnummer ABIN6936403

Der Maus Monoklonal anti-AMACR Antikörper (Klon rAMACR-1864) (ABIN6936403) detektiert spezifisch AMACR in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter AMACR Antikörper (AA 297-394) (ABIN6936403)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AMACR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: RAMACR-1864

Produktdetails anti-AMACR Antikörper

Bindungsspezifität: AA 297-394

Verwendungszweck: Recombinant AMACR / p504S (Prostate Cancer Marker) Antibody

Spezifität: This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK cells. Prostate Adenocarcinoma and human kidney tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: AMACR

Hintergrund:

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

HEK cells. Prostate Adenocarcinoma. Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney and Guinea pig Kidney tissue lysates using AMACR Recomb

Molekulargewicht: 42kDa

Gen-ID: 23600, 508343

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process