SQSTM1 Antikörper (AA 51-150)

Produktnummer ABIN682153

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SQSTM1 in WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Rind (Kuh) und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für SQSTM1 Antikörper (AA 51-150) (ABIN682153)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SQSTM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SQSTM1 Antikörper

Bindungsspezifität: AA 51-150

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SQSTM1/Ubiquitin-binding protein p62

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-SQSTM1 Antikörper (ABIN682153)

Kuo, Weng, Chang, Huang, Lin, Chuang, Lin, Liou, Tai, Lin, Wang: "Altered mitochondrial dynamics and response to insulin in cybrid cells harboring a diabetes-susceptible mitochondrial DNA haplogroup." in: Free radical biology & medicine, Vol. 96, pp. 116-29, (2016) (PubMed).

Xia, Gao, Zhang, Wang, Zhao, Che, Ao, Yang, Wang, Lei: "Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells." in: Cell death discovery, Vol. 2, pp. 15065, (2016) (PubMed).

Cui, Luo, Li, Li, Wang: "Changes of intracellular Ca2+ in quercetin-induced autophagy progression." in: Acta biochimica et biophysica Sinica, Vol. 47, Issue 11, pp. 908-14, (2015) (PubMed).

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." in: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Wang, Gao, Yao, Xie: "Blocking autophagic flux enhances matrine-induced apoptosis in human hepatoma cells." in: International journal of molecular sciences, Vol. 14, Issue 12, pp. 23212-30, (2014) (PubMed).

Details zu SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Andere Bezeichnung: SQSTM1

Hintergrund:

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, SQSTM1, ORCA

Background: Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Gen-ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Autophagie