Tumor Necrosis Factor Antikörper (Tumor Necrosis Factor alpha) (AA 180-230)

Details for Product anti-TNF Antibody No. ABIN677318
Antigen
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Epitop
AA 180-230
82
47
46
44
22
15
14
8
8
7
7
7
7
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
933
362
284
117
112
96
95
56
51
40
26
17
15
6
6
6
5
4
4
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
675
485
78
48
10
6
6
5
2
2
2
2
Klonalität
Polyklonal
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
160
71
35
27
20
11
11
11
11
11
11
11
11
11
11
11
11
11
9
8
7
7
7
7
7
3
2
2
2
2
2
2
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
652
618
419
389
244
125
118
87
71
69
63
59
32
26
25
19
18
16
14
9
8
8
7
6
6
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Optionen
Immunogen KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotyp IgG
Reinigung Purified by Protein A.
Antigen
Andere Bezeichnung Tnf alpha (TNF Antibody Abstract)
Hintergrund

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular

Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf

Gen-ID 21926
UniProt P06804
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Image no. 2 for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
Image no. 3 for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Image no. 4 for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300...
Produkt verwendet in: Lennikov, Mirabelli, Mukwaya, Schaupper, Thangavelu, Lachota, Ali, Jensen, Lagali: "Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis." in: Angiogenesis, Vol. 21, Issue 2, pp. 267-285, 2019 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." in: Cytokine, Vol. 99, pp. 275-280, 2018 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, 2018 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Dong, Zhou, Ji, Pan, Zheng: "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE-/- mice." in: Biochemical and biophysical research communications, Vol. 489, Issue 4, pp. 426-431, 2017 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Mouse (Murine)).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, 2017 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB)

Shi, Yu, Liu, Liu, Zhang, Yang, Chai, Lou, Gao, Zhu: "Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 221-233, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: brain

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rabbit). Weitere Details: Sample: lung

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB) (Probematerial (Species): Mouse (Murine)). Weitere Details: Sample: liver

Bertuglia, Pagliara, Grego, Ricci, Brkljaca-Bottegaro: "Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career." in: BMC veterinary research, Vol. 12, Issue 1, pp. 246, 2016 (PubMed). Von den Autoren verwendete Methode: ELISA (ELISA), ELISA (ELISA)

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF?? by injection of lentivirus?mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015 (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, 2015 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, 2015 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)).

Fang, Li, Sun, Bao, Ren, Lv, Ma: "Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption." in: International journal of molecular sciences, Vol. 14, Issue 5, pp. 10343-54, 2015 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB)

Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, 2015 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB)

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Rat (Rattus)).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)).

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." in: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: liver

'Independent Validation' Siegel
Chargennummer 140113
Validierte Anwendung Immunofluorescence
Positivkontrolle Normal mouse lung tissue
Negativkontrolle Normal mouse adipose tissue
Bewertung Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Primärantikörper
  • Antibody: Tumor Necrosis Factor (TNF)
  • Catalog number: ABIN677318
  • Lot number: 140113
Isotypkontrolle
  • Antibody: Rabbit IgG Isotype
  • Lot number: not available
Sekundärantikörper
  • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
  • Lot number: Not available
Kontrollen
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protokoll
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Anmerkungen Nothing to note.
Validierungsbilder
Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only
Haben Sie etwas anderes gesucht?