Leptin Antikörper (N-Term)

Produktnummer ABIN6745724

This anti-Leptin antibody (ABIN6745724) is a Rabbit Polyclonal antibody detecting Leptin in WB, IHC, IHC (p). Suitable for Human, Mouse, Cow, Horse, Dog, Rabbit, Hamster.

Kurzübersicht für Leptin Antikörper (N-Term) (ABIN6745724)

Target: Leptin (LEP)

Reaktivität: Human, Maus, Rind (Kuh), Pferd, Hund, Kaninchen, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Leptin Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human LEP / Leptin

Homologie: Percent identity by BLAST analysis: Human, Mouse (100%) Rat (92%) Sheep, Goat (90%) Dog, Bovine (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Hamster (100%), Galago, Marmoset, Rat, Opossum (92%), Sheep, Goat (90%), Monkey, Elephant, Zebu, Dog, Cat, Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

Gen-ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process